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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Attenuation of High Glucose-Induced Damage in RPE Cells through p38 MAPK Signaling Pathway Inhibition
doi: 10.3389/fphar.2021.684680
Figure Lengend Snippet: Effects of DMF on p38 MAPK pathway. ARPE-19 cells exposed to normal glucose (NG) or high glucose (HG) w/or w/o DMF (10 μM) for 24 h (A) p38 MAPK was measured by western blot analysis; band densities were quantified by ImageQuantTL software and normalized values were plotted in the histogram shown on the right (*** p < 0.001 vs. NG; † p < 0.05 vs. untreated/HG; n = 6). (B) ARPE-19 cells exposed to normal glucose (NG) or high glucose (HG) were pretreated with SB202190 (10 µM), a p38 MAPK inhibitor for 30 min and then treated with DMF (10 μM) for 24 h. VEGF levels were measured through ELISA. * p < 0.05 vs. NG untreated cells; n = 6. ( C ) MTT analysis: values are expressed as percentage of cell viability in NG untreated cells. * p < 0.05 or ** p < 0.01 vs. NG untreated cells; n = 6.
Article Snippet: Primary antibodies: BAX (sc-493, Santa Cruz Biotechnology, Inc, CA, United States), Bcl-2 (sc-509, Santa Cruz Biotechnology, Inc, CA, United States), COX-2 (sc-19999, Santa Cruz Biotechnology, Inc, CA, United States), iNOS (sc-651, Santa Cruz Biotechnology, Inc, CA, United States), p38 MAPK (#9212, Cell Signaling Technology, United States),
Techniques: Western Blot, Software, Enzyme-linked Immunosorbent Assay